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J Microsc. 2000 Jan;197(Pt 1):1-4.

Wide-field optically sectioning fluorescence microscopy with laser illumination.

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Department of Engineering Science, University of Oxford, Parks Road, Oxford OX1 3PJ, U.K.


We describe an extremely simple method by which optically sectioned fluorescence images may be obtained with conventional microscopes using laser illumination. A one-dimensional grid pattern is introduced into the illumination system, together with a rotating ground glass diffuser. This causes an image of the grid pattern to be projected into the specimen. Images taken at three spatial positions of the grid are processed in a simple manner to provide optically sectioned images of fluorescent specimens.

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