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Clin Biochem. 1999 Oct;32(7):547-55.

Ethanol-induced apoptosis in vitro.

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  • 1Division of Clinical Pharmacology, Sunnybrook and Women's College Health Sciences Centre, Toronto, Ontario, Canada. manuela@srcl.sunnybrook.utoronto.ca

Abstract

OBJECTIVES:

The aim is to study the apoptotic process in a human hepatocyte model for ethanol (EtOH)-induced apoptosis.

DESIGN AND METHODS:

Normal human primary hepatocytes (HPH) and Hep G2 cells were exposed to increasing EtOH. 6000 cells/ sample were analyzed by transmission electron microscopy.

RESULTS:

Apoptotic cells were observed (mmol/L EtOH): 40: 6 +/-0.5%, 60:13 +/- 2% (p < 0.05), 80: 26 +/- 1% (p < 0.001) (vs. control). Two consecutive doses of 80 mmol/L for 24 h each additionally increased apoptosis 55 +/- 3% (p < 0.0001 vs. control and p < 0.001 vs. single dose). In response to this exposure, there is a stronger apoptotic activity in HPH when compared to Hep G2 (p < 0.05).

CONCLUSIONS:

In vitro, EtOH-induced apoptosis is regulated by dose level and the frequency of exposure.

PMID:
10614717
[PubMed - indexed for MEDLINE]
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