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Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):15348-53.

A bZIP factor, TRAB1, interacts with VP1 and mediates abscisic acid-induced transcription.

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  • 1Center for Molecular Biology, Mie University, 1515 Kamihama-cho, Tsu 514-8507, Japan.

Abstract

The transcription factor VP1 regulates maturation and dormancy in plant seeds by activating genes responsive to the stress hormone abscisic acid (ABA). Although activation involves ABA-responsive elements (ABREs), VP1 itself does not specifically bind ABREs. Instead, we have identified and cloned a basic region leucine zipper (bZIP) factor, TRAB1, that interacts with both VP1 and ABREs. Transcription from a chimeric promoter with GAL4-binding sites was ABA-inducible if cells expressed a GAL4 DNA-binding domain::TRAB1 fusion protein. Results indicate that TRAB1 is a true trans-acting factor involved in ABA-regulated transcription and reveal a molecular mechanism for the VP1-dependent, ABA-inducible transcription that controls maturation and dormancy in plant embryos.

PMID:
10611387
PMCID:
PMC24822
[PubMed - indexed for MEDLINE]
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