The essential cofactor TRRAP recruits the histone acetyltransferase hGCN5 to c-Myc

Mol Cell Biol. 2000 Jan;20(2):556-62. doi: 10.1128/MCB.20.2.556-562.2000.

Abstract

The c-Myc protein functions as a transcription factor to facilitate oncogenic transformation; however, the biochemical and genetic pathways leading to transformation remain undefined. We demonstrate here that the recently described c-Myc cofactor TRRAP recruits histone acetylase activity, which is catalyzed by the human GCN5 protein. Since c-Myc function is inhibited by recruitment of histone deacetylase activity through Mad family proteins, these opposing biochemical activities are likely to be responsible for the antagonistic biological effects of c-Myc and Mad on target genes and ultimately on cellular transformation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetyltransferases / metabolism*
  • Adaptor Proteins, Signal Transducing
  • Animals
  • Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
  • Basic-Leucine Zipper Transcription Factors
  • Carrier Proteins / metabolism
  • Catalytic Domain
  • Cell Cycle Proteins
  • Cell Line
  • Cell Transformation, Neoplastic
  • DNA-Binding Proteins / metabolism
  • Dimerization
  • Genes, Essential / genetics
  • Genes, Essential / physiology
  • HeLa Cells
  • Histone Acetyltransferases
  • Histone Deacetylases / metabolism
  • Humans
  • Models, Biological
  • Nuclear Proteins / metabolism*
  • Precipitin Tests
  • Protein Binding
  • Proto-Oncogene Proteins c-myc / antagonists & inhibitors
  • Proto-Oncogene Proteins c-myc / chemistry
  • Proto-Oncogene Proteins c-myc / genetics
  • Proto-Oncogene Proteins c-myc / metabolism*
  • Rats
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Repressor Proteins*
  • Saccharomyces cerevisiae Proteins*
  • Trans-Activators / chemistry
  • Trans-Activators / genetics
  • Trans-Activators / metabolism*
  • Transcription Factors*
  • p300-CBP Transcription Factors

Substances

  • Adaptor Proteins, Signal Transducing
  • Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
  • Basic-Leucine Zipper Transcription Factors
  • Carrier Proteins
  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • MAX protein, human
  • MXD1 protein, human
  • Max protein, rat
  • Myc associated factor X
  • Nuclear Proteins
  • Proto-Oncogene Proteins c-myc
  • Recombinant Fusion Proteins
  • Repressor Proteins
  • Saccharomyces cerevisiae Proteins
  • Trans-Activators
  • Transcription Factors
  • transformation-transcription domain-associated protein
  • Acetyltransferases
  • Histone Acetyltransferases
  • KAT2A protein, human
  • p300-CBP Transcription Factors
  • p300-CBP-associated factor
  • Histone Deacetylases