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Int Immunol. 2000 Jan;12(1):113-7.

Cellular responses to bacterial cell wall components are mediated through MyD88-dependent signaling cascades.

Author information

1
Department of Host Defense, Research Institute for Microbial Diseases, Osaka University and CREST of Japan Science and Technology Corp., 3-1 Yamada-oka, Suita, Osaka 565-0871, Japan.

Abstract

MyD88 is an adaptor molecule essential for signaling via the Toll-like receptor (TLR)/IL-1 receptor family. TLR4 is a member of the TLR family and a point mutation in the Tlr4 gene causes hyporesponsiveness to lipopolysaccharide (LPS) in C3H/HeJ mice. We have previously shown that both TLR4- and MyD88-deficient mice are hyporesponsive to LPS. In this study we examined the responsiveness of these two knockout mice to various bacterial cell wall components. Cells from TLR4-deficient mice responded to several kinds of LPS, peptidoglycan and crude cell wall preparation from Gram-positive bacteria and mycobacterial lysates. In contrast, macrophages and splenocytes from MyD88-deficient mice did not respond to any of the bacterial components we tested. These results show that MyD88 is essential for the cellular response to bacterial cell wall components.

PMID:
10607756
DOI:
10.1093/intimm/12.1.113
[Indexed for MEDLINE]

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