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Biochem Biophys Res Commun. 1999 Dec 29;266(3):729-34.

Tn5: A molecular window on transposition.

Author information

1
Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, Wisconsin, 53706, USA. reznikoff@biochem.wisc.edu

Abstract

DNA transposition is an underlying process involved in the remodeling of genomes in all types of organisms. We analyze the multiple steps in cut-and-paste transposition using the bacterial transposon Tn5 as a model. This system is particularly illuminating because of the existence of structural, genetic, and biochemical information regarding the two participating specific macromolecules: the transposase and the 19-bp sequences that define the ends of the transposon. However, most of the insights should be of general interest because of similarities to other transposition-like systems such as HIV-1 DNA integration into the host genome.

PMID:
10603311
DOI:
10.1006/bbrc.1999.1891
[Indexed for MEDLINE]

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