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Eur J Immunol. 1999 Dec;29(12):4053-9.

Enrichment and detection of live antigen-specific CD4(+) and CD8(+) T cells based on cytokine secretion.

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1
Miltenyi Biotec GmbH Bergisch Gladbach, Germany.

Abstract

Following appropriate antigen-specific stimulation, CD4(+) and CD8(+) T lymphocytes rapidly express cytokines. Based on this stimulation-induced cytokine secretion and using cell surface affinity matrix technology we have developed a new method that permits specific, rapid and efficient detection, isolation and characterization of live antigen-specific CD4(+) and CD8(+) T lymphocytes. The power of this technique is demonstrated here for HLA-A0201-restricted influenza matrix protein peptide 58-66-specific CD8(+) cytotoxic T lymphocytes, influenza A virus- and recombinant tetanus toxin C fragment-specific Th1 cells and tetanus toxoid-specific Th2 cells.

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