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FEBS Lett. 1999 Dec 10;463(1-2):160-4.

Cloning of bovine RANTES mRNA and its expression and regulation in ovaries in the periovulatory period.

Author information

1
Institute of Anatomy, University of Leipzig, Liebigstrasse 13, Leipzig, Germany. ausg@medizin.uni-leipzig.de

Abstract

RANTES may be one of the chemoattractants involved in stimulating eosinophils and macrophages to migrate selectively into bovine dominant follicles and into developing corpora lutea. We sequenced a 736 bp fragment of the bovine RANTES mRNA encoding the complete protein and defined the ovarian source of RANTES mRNA. As demonstrated by competitive RT-PCR, follicle-derived macrophages showed a 100-1000 times higher RANTES mRNA level compared to unpurified granulosa cells or follicle-derived fibroblasts. By means of in situ hybridization, RANTES mRNA positive macrophages were located in the former thecal layer of the developing corpora lutea.

PMID:
10601659
DOI:
10.1016/s0014-5793(99)01599-9
[Indexed for MEDLINE]
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