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FEBS Lett. 1999 Dec 10;463(1-2):72-6.

Drosophila melanogaster protein phosphatase inhibitor-2: identification of a site important for PP1 inhibition.

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Medical Research Council Protein Phosphorylation Unit, Department of Biochemistry, MSI/WTB Complex, University of Dundee, Dundee, UK.


A database search with human protein phosphatase inhibitor-2 (I-2) identified a Drosophila melanogaster cDNA that encoded a protein identical in length and sharing 39% amino acid identity (58% similarity) with human I-2. The mRNA encoding this protein is expressed in both sexes and throughout development, unlike Drosophila inhibitor-t. The bacterially expressed protein was a specific inhibitor of protein phosphatase 1 with an IC(50) of <1 nM, confirming that it is the Drosophila homologue of mammalian inhibitor-2. Mutation of Phe residues conserved in I-2 from lower and higher eukaryotes showed that Phe-33 is important for inhibition of PP1c.

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