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Exp Parasitol. 1999 Dec;93(4):225-30.

Leishmania donovani: characterization and expression of ORFF, a gene amplified from the LDI locus.

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  • 1School of Life Science, Jawaharlal Nehru University, New Delhi, 110067, India.

Abstract

The LD1 locus is a 27.5-kb region of chromosome 35 that is conserved among all species of Leishmania and is amplified in several different isolates. Here, we report the genomic distribution of ORFF, a gene from the LD1 region, and its expression at the RNA and protein levels in two Indian isolates of Leishmania donovani. In both of these isolates, ORFF was present as a single copy on chromosome 35. Densitometric analysis of ORFF mRNA abundance revealed relative abundance of 0.2 and 1.0 in AG83 and S-Lal, respectively. Antiserum against recombinant ORFF protein detected a protein of the predicted size ( approximately 34 kDa) in both strains. The protein is most abundant in mid-log-phase promastigotes and has a nuclear localization. The ORFF protein is preferentially expressed in L. donovani amastigotes but, in contrast, is expressed at higher levels in L. major promastigotes.

PMID:
10600448
DOI:
10.1006/expr.1999.4453
[PubMed - indexed for MEDLINE]

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