Abstract
Cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) is a CD28 homologue which down-modulate T cell responses rather than augment them. To investigate its biological role in feline immune system, we cloned and sequenced full-length feline CTLA-4 (fCTLA-4) cDNA by RT-PCR from pokeweed mitogen stimulated peripheral blood lymphocytes. The fCTLA-4 contains an open reading frame of 669 nucleotides, coding for a polypeptide of 223 amino acids. The predicted fCTLA-4 amino acids sequence shows the homology of 86.6%, 87.0%, and 76.2% with human, bovine, and murine molecules respectively. The hexapeptide motif (MYPPPY) within the extra-cellular domain of CTLA-4 molecule, which is believed to be responsible for interaction with the B7 family members, is completely conserved in all the species.
MeSH terms
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Abatacept
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Amino Acid Motifs / genetics
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Amino Acid Sequence
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Animals
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Antigens, CD
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Antigens, Differentiation / chemistry
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Antigens, Differentiation / genetics*
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Antigens, Differentiation / physiology
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Base Sequence
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CTLA-4 Antigen
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Cats / genetics*
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Cats / immunology
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Cloning, Molecular
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DNA / chemistry
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DNA Primers / chemistry
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DNA, Complementary / chemistry*
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DNA, Complementary / genetics
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Immunoconjugates*
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Molecular Sequence Data
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RNA / chemistry
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RNA / isolation & purification
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Alignment
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
Substances
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Antigens, CD
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Antigens, Differentiation
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CTLA-4 Antigen
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CTLA4 protein, human
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DNA Primers
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DNA, Complementary
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Immunoconjugates
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RNA
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Abatacept
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DNA
Associated data
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GENBANK/AF153202
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GENBANK/X05719