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Nat Biotechnol. 1999 Dec;17(12):1179-83.

Myogenic expression of an injectable protease-resistant growth hormone-releasing hormone augments long-term growth in pigs.

Author information

1
Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030, USA. ada@bcm.tmc.edu
2
Baylor Coll Med, Houston, TX

Abstract

Ectopic expression of a new serum protease-resistant porcine growth hormone-releasing hormone, directed by an injectable muscle-specific synthetic promoter plasmid vector (pSP-HV-GHRH), elicits growth in pigs. A single 10 mg intramuscular injection of pSP-HV-GHRH DNA followed by electroporation in three-week-old piglets elevated serum GHRH levels by twofold to fourfold, enhanced growth hormone secretion, and increased serum insulin-like growth factor-I by threefold to sixfold over control pigs. After 65 days the average body weight of the pigs injected with pSP-HV-GHRH was approximately 37% greater than the placebo-injected controls and resulted in a significant reduction in serum urea concentration, indicating a decrease in amino acid catabolism. Evaluation of body composition indicated a uniform increase in mass, with no organomegaly or associated pathology.

PMID:
10585714
DOI:
10.1038/70718
[Indexed for MEDLINE]

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