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Appl Environ Microbiol. 1999 Dec;65(12):5619-23.

Engineering of a stable whole-cell biocatalyst capable of (S)-styrene oxide formation for continuous two-liquid-phase applications.

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1
Institute of Biotechnology, Swiss Federal Institute of Technology Zurich, 8093 Zurich, Switzerland.

Abstract

Recombinant strains of Pseudomonas putida KT2440 carrying genetic expression cassettes with xylene oxygenase- and styrene monooxygenase-encoding genes on their chromosomes could be induced in shaking-flask experiments to specific activities that rivaled those of multicopy-plasmid-based Escherichia coli recombinants. Such strains maintained the introduced styrene oxidation activity in continuous two-liquid-phase cultures for at least 100 generations, although at a lower level than in the shaking-flask experiments. The data suggest that placement of target genes on the chromosome might be a suitable route for the construction of segregationally stable and highly active whole-cell biocatalysts.

PMID:
10584030
PMCID:
PMC91770
[Indexed for MEDLINE]
Free PMC Article
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