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Acta Microbiol Pol. 1999;48(2):123-9.

Cloning of the Dam methyltransferase gene from Haemophilus influenzae bacteriophage HP1.

Author information

1
Institute of Microbiology, Warsaw University, Poland.

Abstract

The putative product of orf13 from the genome of Haemophilus influenzae HP1 bacteriophage shows homology only to bacteriophage T1 Dam methyltransferase, and a weak similarity to the conserved amino acids sequence motifs characteristic of m6A-methyltransferases. Especially interesting is lack of characteristic motif I responsible for binding of S-adenosylmethionine. Despite this fact, a DNA sequence of HP1 bacteriophage of Haemophilus influenzae encoding methyltransferase activity was cloned and expressed in Escherichia coli using pMPMT4 omega expression vector. The cloned methyltransferase recognizes the sequence 5'-GATC-3' and methylates an adenine residue. The enzyme methylates both double- and single-stranded DNA substrates.

PMID:
10581668
[Indexed for MEDLINE]

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