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J Gen Virol. 1999 Nov;80 ( Pt 11):2861-2865. doi: 10.1099/0022-1317-80-11-2861.

Free thiol groups are essential for infectivity of human cytomegalovirus.

Author information

1
Department of Virology, SMI/Karolinska Institute, 171 82 Solna, Sweden 1.
2
Department of Immunology, Microbiology, Pathology and Infectious Disease, Karolinska Institute, Box 12 773, 112 96 Stockholm, Sweden 2.

Abstract

The membrane-impermeable thiol blocker 5'5-dithiobis 2- nitrobenzoic acid (DTNB) blocked infectivity of human cytomegalovirus (CMV) although the virus still bound to cells. DTNB-treated CMV regained 65% of its infectivity after incubation with the disulfide bond-reducing agent dithiothreitol. These observations suggest that free thiol groups on CMV are required for infectivity and may participate in disulfide bond formation during virus entry.

PMID:
10580047
DOI:
10.1099/0022-1317-80-11-2861
[Indexed for MEDLINE]

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