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Diagn Microbiol Infect Dis. 1999 Oct;35(2):105-8.

Multiplex PCR for rapid and differential diagnosis of Mycoplasma pneumoniae and Chlamydia pneumoniae in respiratory infections.

Author information

1
Unité Mixte de Recherche 7565 UHP-CNRS-Laboratoire de Bactériologie-Virologie, Faculté de Médecine, Vandoeuvre-lès-Nancy, France.

Abstract

A duplex polymerase chain reaction (PCR) was developed for the simultaneous detection of Chlamydia pneumoniae and Mycoplasma pneumoniae. A study of 163 respiratory specimens from in-patients of the "Centre Hospitalier et Universitaire de Nancy" showed the good sensitivity of this duplex PCR allowing the detection of C. pneumoniae and M. pneumoniae from 8 and 13 patients, respectively, whereas the culture was negative for C. pneumoniae for all the samples and positive for M. pneumoniae only in 9 cases. The value of these results has been confirmed by running on the same samples specific nested PCRs for these two microorganisms that gave the same results. Thus, the proposed duplex amplification technique may facilitate the diagnosis of infection by these two agents that are difficult to isolate.

PMID:
10579089
DOI:
10.1016/s0732-8893(99)00070-x
[Indexed for MEDLINE]

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