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Zhonghua Gan Zang Bing Za Zhi. 1999 Sep;7(3):140-1.

[A study on expression of autotaxin mRNA in hepatocellular carcinoma].

[Article in Japanese]

Author information

1
Department of Gastroenterology, First Affiliated Hospital, Nanjing Medical University.

Abstract

OBJECTIVE:

To know if ATX is expressed in hepatocellular carcinoma.

METHODS:

7721 hepatoma cell lines in culture, 5 normal liver tissues, and 32 histologically verified HCC specimens were obtained. Semiquantitative reverse transcription polymerase chain reaction was used to detect mRNA expression of ATX.

RESULTS:

The cDNA sequence of RT-PCR product of 7721 hepatoma cell lines was measured, it showed that nucleotides measured were 332 bp. ATX cDNA nucleotide sequence in HCC was found to share 99.7% homology with that in Melanoma. ATX was expressed in all of the 32 HCC and 5 normal liver tissues. The mean expression level of ATX gene in HCC samples was higher than that in normal liver tissues (0.68 +/- 0.1 vs 0.3 +/- 0.08, P < 0.01).

CONCLUSION:

ATX gene was found to be in existence in HCC and normal liver tissues. The homology of ATX cDNA sequence between HCC and human Melanoma was 99.7%, and ATX may play an important role in HCC metastatic mechanism.

PMID:
10572678
[Indexed for MEDLINE]

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