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Zhonghua Gan Zang Bing Za Zhi. 1999 Sep;7(3):138-9.

[The correlation between integration of HBV X, S, Pre-S, C gene and the expression of oncogenes/tumor suppressor genes in primary hepatocellular carcinoma].

[Article in Japanese]

Author information

1
Shandong Medical University, Jinan.

Abstract

OBJECTIVE:

To study the relation between the integration of HBV X, S, Pre-S and C genes and the expression of oncogenes/tumor suppressor genes in hepatocellular carcinoma(HCC).

METHODS:

To prepare the Dig-HBV probes with random priming method, to detect the existing status of HBV DNA and the integration rates of HBV X, S, Pre-S and C genes by Southern blot and dot blot hybridization, and to examine the expression of oncogenes and tumor suppressor genes by immunohistochemistry in HCC.

RESULTS:

The integration rates of HBV X, S, Pre-S and C genes in HCC were 87.5%(28/32), 62.5% (20/32), 62.5%(20/32) and 25.0%(8/32), respectively. The expression rates of p62myc, p21ras, p53 and p16 proteins were 81.2%(26/32), 50.0%(16/32), 43.8%(14/32), and 31.3(10/32), respectively. Statistical analysis showed that the expression of p62myc, p21ras and variant p53 proteins was obviously higher in HCC group with X gene than in free-X gene HCC group and that the expression of p62myc protein was obviously higher in HCC group with Pre-S gene than in free-Pre-S HCC group.

CONCLUSION:

The integration of HBV X or Pre-S gene with cellular chromosome can activate myc, ras oncogenes and inactivate the p53 tumor suppressor gene.

PMID:
10572677
[Indexed for MEDLINE]

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