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FEMS Microbiol Lett. 1999 Dec 1;181(1):153-7.

Growth and cytotoxic activity by Mycobacterium ulcerans in protein-free media.

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Laboratoire de Biochimie et Biologie Cellulaire, Facult├ęs Universitaires Notre Dame de la Paix, Rue de Bruxelles 61, B-5000, Namur, Belgium.


The pathogenic slow-growing Mycobacterium ulcerans has, until now, been cultured in liquid media containing albumin. Here, we report the first description of use of Sauton medium and modified Reid medium, two protein-free media, in which M. ulcerans was able to grow and produce its toxin, a major virulence factor of this environmental organism which causes a skin disease commonly called Buruli ulcer. These results suggest that Sauton and modified Reid may be useful for certain fields of M. ulcerans research requiring protein-free growth conditions.

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