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Virology. 1999 Nov 25;264(2):436-40.

Integrin alphaVbeta5 is not involved in adeno-associated virus type 2 (AAV2) infection.

Author information

1
Hematology Branch, National Heart, Lung and Blood Institute, Bethesda, Maryland, 20892, USA. QuiJ@nih.gov

Abstract

alphaVbeta5 integrin was recently proposed as a coreceptor for adeno-associated virus type 2 (AAV2) infection (Summerford et al., 1999, Nat. Med. 5, 78-82), based mainly on the direct binding of AAV2 to denatured beta5 by virus overlay assay. In studies using purified natural or recombinant human integrin alphaVbeta5 we were unable to demonstrate AAV2 binding, either by virus overlay or by liquid binding assay. Furthermore, neither purified integrin alphaVbeta5, nor RGD peptides, nor functional blocking monoclonal antibody blocked rAAV2 transduction. These data strongly suggest that integrin alphaVbeta5 is not involved in AAV2 infection.

PMID:
10562505
DOI:
10.1006/viro.1999.0010
[Indexed for MEDLINE]
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