Format

Send to

Choose Destination
See comment in PubMed Commons below
Nat Cell Biol. 1999 Oct;1(6):358-61.

De novo generation of a PrPSc-like conformation in living cells.

Author information

1
Howard Hughes Medical Institute, Department of Molecular Genetics and Cell Biology, University of Chicago, 5841 S. Maryland Avenue MC1028, Chicago, Illinois 60637, USA.

Abstract

Conformational conversion of the cellular PrPC protein to PrPSc is a central aspect of the prion diseases, but how PrP initially converts to this conformation remains a mystery. Here we show that PrP expressed in the yeast cytoplasm, instead of the endoplasmic reticulum, acquires the characteristics of PrPSc, namely detergent insolubility and a distinct pattern of protease resistance. Neuroblastoma cells cultured under reducing, glycosylation-inhibiting conditions produce PrP with the same characteristics. We therefore describe what is, to our knowledge, the first conversion of full-length PrP in a heterologous system, show the importance of reducing and deglycosylation conditions in PrP conformational transitions, and suggest a model for initiating events in sporadic and inherited prion diseases.

PMID:
10559963
DOI:
10.1038/14053
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group
    Loading ...
    Support Center