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Science. 1999 Nov 12;286(5443):1362-5.

The protein kinase p90 rsk as an essential mediator of cytostatic factor activity.

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1
Department of Molecular Pharmacology, Stanford University School of Medicine, Stanford, CA 94305-5332, USA.

Abstract

Persistent activation of p42 mitogen-activated protein kinase (p42 MAPK) during mitosis induces a "cytostatic factor" arrest, the arrest responsible for preventing the parthenogenetic activation of unfertilized eggs. The protein kinase p90 Rsk is a substrate of p42 MAPK; thus, the role of p90 Rsk in p42 MAPK-induced mitotic arrest was examined. Xenopus laevis egg extracts immunodepleted of Rsk lost their capacity to undergo mitotic arrest in response to activation of the Mos-MEK-1-p42 MAPK cascade of protein kinases. Replenishing Rsk-depleted extracts with catalytically competent Rsk protein restored the ability of the extracts to undergo mitotic arrest. Rsk appears to be essential for cytostatic factor arrest.

PMID:
10558991
[Indexed for MEDLINE]
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