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Biochem Biophys Res Commun. 1999 Nov 19;265(2):285-90.

Identification and characterization of a transcriptional silencer upstream of the human BRCA2 gene.

Author information

1
Division of Biomedical Sciences, Department of Microbiology, Meharry Medical College, Nashville, Tennessee, 37208, USA.

Abstract

Expression of the breast cancer susceptibility tumor-suppressor protein BRCA2, a protein potentially involved in DNA recombination repair, is tightly regulated throughout development. We have identified a transcriptional silencer at the distal end of the human BRCA2 gene promoter. This silencer is involved in the negative regulation of the expression of this gene in breast cell lines tested but not in HeLa or HepG2 cells. The 221-base-pair silencer region is characterized by a full-length Alu-repeat. Presence of specific BRCA2 silencer-binding proteins in the breast cell extracts indicates the potential regulation of BRCA2 gene expression by these proteins.

PMID:
10558858
PMCID:
PMC3100288
DOI:
10.1006/bbrc.1999.1652
[Indexed for MEDLINE]
Free PMC Article

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