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Clin Chim Acta. 1999 Nov;289(1-2):57-68.

A modified cyanide-nitroprusside method for quantifying urinary cystine concentration that corrects for creatinine interference.

Author information

1
Department of Biochemistry & Molecular Biology, and Kidney Stone Program, Department of Medicine, University of Chicago, Chicago, IL 60637, USA. ynakagaw@medicine.bsd.uchicago.edu

Abstract

Cystinuria, an inherited disease, is clinically diagnosed by detecting cystine in urine. A colorimetric method using sodium cyanide and sodium nitroprusside is a simple qualitative test used to detect cystinuria. Several colorimetric methods have been proposed for quantitative analysis of cystine; however, we found that none of them were satisfactory because the results were not reproducible. The causes of non-reproducible results were: (1) insufficient reduction time for conversion of cystine to cysteine, and (2) the interference of creatinine. In this report, we present a method to quantitate cystine in urine. We also found that ascorbic acid and ferric chloride, but not zinc chloride, interfered with the color reaction. Using this method, 15 normal urine samples (10 males and 5 females) and 12 cystine stone forming patients' (5 males and 7 females) urine were analyzed. The method was compared to commercially available urine controls. Only captopril showed a dose dependent response and color intensity at 521 nm. Thiola and D-penicillamine showed little effect on cystine determination.

PMID:
10556653
[Indexed for MEDLINE]

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