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Mol Diagn. 1999 Sep;4(3):169-83.

In situ hybridization-theory and practice.

Author information

1
Department of Pathology, Division of Molecular Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

Abstract

In situ hybridization is a technique to determine and localize target nucleic acids in morphologically preserved tissue sections. Recent advances in methods have greatly increased the sensitivity of the technique, and it is currently possible to detect extremely few copies of any given target sequence with nonisotopic methods. In this teaching review, we integrate theoretical background, technical considerations, and guidelines for usage for this important component of molecular diagnosis.

PMID:
10553018
DOI:
10.154/MODI00400169
[Indexed for MEDLINE]

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