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J Hum Virol. 1999 Sep-Oct;2(5):296-307.

The La protein from human liver cells interacts specifically with the U-rich region in the hepatitis C virus 3' untranslated region.

Author information

1
Department of Medical Biochemistry and Microbiology, Biomedical Center, Uppsala University, Sweden.

Abstract

OBJECTIVE:

To identify cellular factors in human liver that interact with hepatitis C virus (HCV) 5' and 3' untranslated regions (UTRs) and therefore possibly are involved in the regulation of HCV transcription or translation.

METHODS:

We prepared cytoplasmic extracts from human liver biopsy samples and fractionated cellular factors on ion exchange columns. The various fractions from ion exchange columns were subjected to ultraviolet (UV) cross-linking with radiolabeled HCV 5' and 3' UTR RNA probes.

RESULTS:

Two major 45- and 46-kd proteins that interacted specifically with the HCV 5' and 3' UTR were identified. Using Western blot and immunoprecipitation, these proteins were identified as the La antigen.

CONCLUSION:

Our results demonstrate that the La protein from human liver biopsy cells interacts specifically with the U-rich region in the positive strand of the HCV 3' UTR.

PMID:
10551736
[Indexed for MEDLINE]

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