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Clin Diagn Lab Immunol. 1999 Nov;6(6):983-5.

Increased levels of epsilon and gamma isoforms of 14-3-3 proteins in cerebrospinal fluid in patients with Creutzfeldt-Jakob disease.

Author information

1
Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan. htakahas@nih.go.jp

Abstract

We established four hybridoma cell lines producing monoclonal antibodies (MAbs) against 14-3-3 proteins. Immunoblot analysis revealed that epsilon and gamma isoforms were specifically increased in premortem cerebrospinal fluid samples from patients with sporadic Creutzfeldt-Jakob disease. Furthermore, dot immunoblot analysis showed that MAbs were more specific for native antigen than polyclonal antibodies were.

PMID:
10548598
PMCID:
PMC95810
[Indexed for MEDLINE]
Free PMC Article

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