Format

Send to

Choose Destination
Crit Care Med. 1999 Oct;27(10):2184-7.

Human vascular endothelial cells produce tumor necrosis factor-alpha in response to proinflammatory cytokine stimulation.

Author information

1
Department of Obstetrics and Gynecology, University of Helsinki, Finland.

Abstract

OBJECTIVE:

To determine whether human vascular endothelial cells produce tumor necrosis factor-alpha (TNF-alpha) after stimulation with proinflammatory cytokines and bacterial lipopolysaccharides (LPS).

DESIGN:

Prospective, in vitro repeated-measurements analysis of cellular responses.

SETTING:

Research laboratory in an academic medical center.

SUBJECTS:

Human umbilical vein endothelial cells (HUVECs).

INTERVENTIONS:

HUVECs were incubated with interferon-gamma (IFN-gamma), interleukin-1beta (IL-1beta), and LPS, or their different combinations for 2 to 48 hrs.

MEASUREMENTS AND MAIN RESULTS:

TNF-alpha was measured by time-resolved immunofluorometric assay. Unstimulated HUVECs did not produce detectable amounts of TNF-alpha, but IFN-gamma, IL-1beta, and LPS when added together induced TNF-alpha production of HUVECs in a time-dependent manner. Immunofluorescent staining confirmed that the TNF-alpha was produced by endothelial cells. IFN-gamma, IL-1beta, or LPS alone did not induce TNF-alpha production, whereas IFN-gamma and IL-1beta in combination were able to induce TNF-alpha production to some extent, and the production could be further increased with LPS. TNF-alpha messenger RNA expression was detected with reverse transcriptase-coupled polymerase chain reaction in stimulated, but not in unstimulated, HUVECs.

CONCLUSIONS:

HUVECs are capable of producing TNF-alpha after proinflammatory cytokine stimulation and may therefore contribute to the increased amount of TNF-alpha found in pathologic states such as septic shock.

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wolters Kluwer
Loading ...
Support Center