Abstract
Sodium/calcium(-potassium) exchangers (NCX and NCKX) are critical for the rapid extrusion of calcium, which follows the stimulation of a variety of excitable cells. To further understand the mechanisms of calcium regulation in signaling, we have cloned a Drosophila sodium/calcium-potassium exchanger, Nckx30C. The overall deduced protein topology for NCKX30C is similar to that of mammalian NCKX, having five membrane-spanning domains in the NH(2) terminus separated from six at the COOH-terminal end by a large intracellular loop. We show that NCKX30C functions as a potassium-dependent sodium/calcium exchanger, and is not only expressed in adult neurons as was expected, but is also expressed during ventral nerve cord development in the embryo and in larval imaginal discs. Nckx30C is expressed in a dorsal-ventral pattern in the eye-antennal disc in a pattern that is similar to, but broader than that of wingless, suggesting that large fluxes of calcium may be occurring during imaginal disc development. Nckx30C may not only function in the removal of calcium and maintenance of calcium homeostasis during signaling in the adult, but may also play a critical role in signaling during development.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Antiporters*
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Base Sequence
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Body Patterning
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Calcium Signaling*
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Carrier Proteins / antagonists & inhibitors
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Carrier Proteins / chemistry
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Carrier Proteins / genetics*
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Carrier Proteins / metabolism*
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Cell Line
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Chromosomes / genetics
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Cloning, Molecular
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Drosophila Proteins*
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Drosophila melanogaster / embryology
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Drosophila melanogaster / genetics*
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Drosophila melanogaster / growth & development
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Drosophila melanogaster / metabolism
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Eye / cytology
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Eye / embryology
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Eye / growth & development
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Eye / metabolism
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Homeostasis
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Larva / growth & development
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Larva / metabolism
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Molecular Sequence Data
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Monensin / pharmacology
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Nervous System / cytology
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Nervous System / embryology
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Nervous System / growth & development
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Nervous System / metabolism
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Photoreceptor Cells, Invertebrate / embryology
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Photoreceptor Cells, Invertebrate / growth & development
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Photoreceptor Cells, Invertebrate / metabolism
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RNA, Messenger / analysis
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RNA, Messenger / genetics
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Sequence Alignment
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Sequence Homology, Amino Acid
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Sodium-Calcium Exchanger*
Substances
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Antiporters
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Calx protein, Drosophila
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Carrier Proteins
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Drosophila Proteins
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RNA, Messenger
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Sodium-Calcium Exchanger
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potassium-dependent sodium-calcium exchanger
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Monensin