Format

Send to

Choose Destination
EMBO J. 1999 Nov 1;18(21):6169-76.

ATP- and ADP-dnaA protein, a molecular switch in gene regulation.

Author information

1
Max-Planck-Institut für Molekulare Genetik, D-14195 Berlin-Dahlem, Germany.

Abstract

DnaA protein functions by binding to asymmetric 9mer DNA sites, the DnaA boxes. ATP-DnaA and ADP-DnaA bind to 9mer DnaA boxes with equal affinity, but only ATP-DnaA protein binds in addition to an as yet unknown 6mer site, the ATP-DnaA box AGATCT, or a close match to it. ATP-DnaA protein binding to ATP-DnaA boxes is restricted to sites located in close proximity to DnaA boxes, suggesting that protein-protein interaction is required for its stabilization. We show that ATP-DnaA represses dnaA transcription much more efficiently than ADP-DnaA. DnaA is thus a regulatory molecule that, depending on the adenosine nucleotide bound, can bind to different sequences and thereby fulfill distinct functions.

PMID:
10545126
PMCID:
PMC1171680
DOI:
10.1093/emboj/18.21.6169
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center