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Immunogenetics. 1999 Oct;50(1-2):60-6.

Identification and characterization of a fish natural resistance-associated macrophage protein (NRAMP) cDNA.

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1
Cell Biology and Immunology Group, Wageningen Institute of Animal Sciences, Wageningen Agricultural University, P.O. Box 338, 6700 AH Wageningen, The Netherlands.

Abstract

The mouse Lsh/Ity/Bcg locus regulates natural resistance to intracellular pathogens, and the Nramp1 gene was isolated as its candidate. Nramp is part of a small family of at least two genes, Nramp1 and Nramp2. In the present study, a full-length cDNA for carp NRAMP has been isolated and characterized. Nucleotide and predicted amino acid sequence analysis indicate that the carp NRAMP encodes a 548 amino acid membrane protein with 12 putative transmembrane domains, two N-linked glycosylation sites, and an evolutionarily conserved consensus transport motif. The peptide sequence identity among carp and human NRAMP2 is 78%, and 65% with human NRAMP1. Reverse transcription-polymerase chain reaction revealed that carp NRAMP is ubiquitously expressed. Phylogenetic analysis, using neighbor-joining, showed that the carp NRAMP protein clustered together with mammalian NRAMP2 proteins.

PMID:
10541807
[Indexed for MEDLINE]
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