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Nucleic Acids Res. 1999 Nov 15;27(22):4305-13.

Compilation and analysis of sigma(54)-dependent promoter sequences.

Author information

1
Departamento de Reconocimiento Molecular y Bioestructura, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos 62271, México.

Abstract

Promoters recognized by the RNA-polymerase with the alternative sigma factor sigma(54) (Esigma54) are unique in having conserved positions around -24 and -12 nucleotides upstream from the transcriptional start site, instead of the typical -35 and -10 boxes. Here we compile 186 -24/-12 promoter sequences reported in the literature and generate an updated and extended consensus sequence. The use of the extended consensus increases the probability of identifying genuine -24/-12 promoters. The effect of several reported mutations at the -24/-12 elements on RNA-polymerase binding and promoter strength is discussed in the light of the updated consensus.

PMID:
10536136
PMCID:
PMC148710
DOI:
10.1093/nar/27.22.4305
[Indexed for MEDLINE]
Free PMC Article

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