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Biochem Biophys Res Commun. 1999 Oct 22;264(2):343-7.

Loss of molecular interaction between cytochrome c and cardiolipin due to lipid peroxidation.

Author information

1
Gifu International Institute of Biotechnology, Institute of Applied Biochemistry, Yagi Memorial Park, Mitake, Gifu, 505-0116, Japan.

Abstract

To explore the molecular mechanism underlying the translocation of cytochrome c from the mitochondrial inner membrane to the cytosol during apoptosis, we analyzed the molecular interaction between cytochrome c and cardiolipin (CL) by (1)H NMR spectroscopy. Bovine heart CL induced a drastic broadening of the linewidth of the downfield signals at 31.4 and 34.2 ppm assigned to the heme methyl group-3 and -8, respectively, of horse heart cytochrome c. In contrast, CL mono- and dihydroperoxides were less active in broadening the signals than CL, and CL trihydroperoxides induced almost no broadening of their linewidth. This finding suggests that the peroxidation of CL induces a release of cytochrome c from mitochondria into the cytosol, which release induces apoptosis in the cells.

PMID:
10529366
DOI:
10.1006/bbrc.1999.1410
[Indexed for MEDLINE]

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