Format

Send to

Choose Destination
Mol Cell Biol Res Commun. 1999 Jul;2(1):58-63.

Interactions between Candida albicans and the human extracellular matrix component tenascin-C.

Author information

1
Department of Microbiology and Immunology, Texas Tech University Health Sciences Center, Lubbock 79430, USA.

Abstract

Tenascins are large multimeric proteins that contain repeated structural motifs that include epidermal growth factor (EGF)-like repeats, fibronectin type III repeats and a globular fibrinogen-like domain, and are involved in tissue and organ morphogenesis, as well as in adhesion and migration of cells. C. albicans germ-tubes, but not blastospores, were able to bind to soluble human tenascin-C as revealed by an indirect immunofluorescence assay. However, materials present in cell wall extracts from both morphologies attached to tenascin-C immobilized in wells of a microtiter plate. The binding specificity was demonstrated by the inhibitory effect of antibodies against C. albicans cell wall components and an anti-tenascin antibody, but not anti-laminin antibody. Fibronectin, but not fibrinogen, inhibited binding, thus indicating a role of the fibronectin type III repeats in the interaction between the fungus and tenascin-C. Binding of C. albicans cell wall materials to tenascin was RGD- and divalent cation-independent.

PMID:
10527893
DOI:
10.1006/mcbr.1999.0152
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center