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Exp Cell Res. 1999 Nov 1;252(2):273-80.

Isolation and characterization of a near-haploid human cell line.

Author information

1
Department of Physiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, Massachusetts, 02111, USA.

Abstract

Mammalian somatic cells are usually diploid. Occasional rare human tumors have been shown to have a hypodiploid karyotype. We have isolated a near-haploid subclone (P1-55) from a heterogeneous human leukemia cell line, KBM-7. These near-haploid cells have approximately half the human diploid DNA content and have a haploid karyotype except for a disomy of chromosome 8 (25, XY, +8, Ph(+)). This cell line maintains a majority of cells with a near-haploid karyotype for at least 12 weeks in culture. By serial subcloning, we have isolated near-haploid subclones that maintain ploidy for at least 8 months in culture. Near-haploid cells can also be efficiently isolated from mixed ploidy cultures by size selection. The availability of this human near-haploid cell line should facilitate the genetic analysis of cultured human cells.

PMID:
10527618
DOI:
10.1006/excr.1999.4656
[Indexed for MEDLINE]

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