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Fertil Steril. 1999 Oct;72(4):713-9.

Expression of leukemia inhibitory factor and its receptor in preimplantation embryos.

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1
Department of Obstetrics and Gynecology, National Taiwan University Hospital, Taipei.

Abstract

OBJECTIVE:

To examine the expression of leukemia inhibitory factor (LIF) and its receptor (LIF-R) transcripts in human and murine preimplantation embryos.

DESIGN:

Prospective study.

SETTING:

University medical center.

PATIENT(S):

Human oocytes were obtained from patients undergoing IVF treatment. Two-cell murine embryos were obtained from ICR strain mice.

INTERVENTION(S):

Second-day intracytoplasmic sperm injection procedures were performed on oocytes that failed to be fertilized by IVF. Embryos were cultured to various stages and collected for study.

MAIN OUTCOME MEASURE(S):

The transcript levels of LIF and LIF-R in these embryos were examined and semiquantitated using single-cell reverse transcription-polymerase chain reaction methodology.

RESULT(S):

Leukemia inhibitory factor and LIF-R transcripts were detectable in most human preimplantation embryos (30 of 34 and 31 of 34 embryos showed LIF and LIF-R messenger RNA, respectively). There was a trend toward decreased expression of both transcripts in embryos at the four-cell stage and in embryos in which growth had been arrested for 24-48 hours. The expression of LIF and LIF-R genes in murine embryos was inconsistent.

CONCLUSION(S):

Preimplantation human embryos express LIF and LIF-R messenger RNA. It is suggested that LIF may be able to affect embryo development through its action at stages before implantation in an autocrine or paracrine manner.

PMID:
10521116
[Indexed for MEDLINE]
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