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Nature. 1999 Sep 23;401(6751):345-50.

Casein kinase I transduces Wnt signals.

Author information

1
Center for Developmental Biology, Department of Molecular Biology, UT Southwestern Medical Center, Dallas, Texas 75235-9133, USA. graff02@utsw.swmed.edu

Abstract

The Wnt signalling cascade is essential for the development of both invertebrates and vertebrates, and is altered during tumorigenesis. Although a general framework for Wnt signalling has been elucidated, not all of the components have been identified. Here we describe a serine kinase, casein kinase I (CKI), which was isolated by expression cloning in Xenopus embryos. CKI reproduces several properties of Wnt signals, including generation of complete dorsal axes, stabilization of beta-catenin and induction of genes that are direct targets of Wnt signals. Dominant-negative forms of CKI and a pharmacological blocker of CKI inhibited Wnt signals in Xenopus. Inhibiting CKI in Caenorhabditis elegans generated worms with a mom phenotype, indicative of a loss of Wnt signals. In addition, CKI bound to and increased the phosphorylation of dishevelled, a known component of the Wnt pathway. These data indicate that CKI may be a conserved component of the Wnt pathway.

PMID:
10517632
DOI:
10.1038/43830
[Indexed for MEDLINE]
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