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J Antimicrob Chemother. 1999 Sep;44(3):377-80.

Molecular characterization of a multiply resistant Klebsiella pneumoniae encoding ESBLs and a plasmid-mediated AmpC.

Author information

1
Center for Research in Anti-Infectives and Biotechnology, Department of Pediatrics, Creighton University School of Medicine and Methodist Hospital, Omaha, NE 68178, USA. ndhanson@creighton.edu

Abstract

Organisms encoding multiple antibiotic resistance genes are becoming increasingly prevalent. In this report we describe a multiply resistant Klebsiella pneumoniae which possesses at least five different beta-lactamase genes. Isoelectric focusing, polymerase chain reaction and restriction fragment length polymorphism analysis identified TEM-1, multiple SHVs, OXA-9 and a plasmid-mediated ampC, beta-lactamase. Furthermore, Southern analysis and conjugation experiments established that most of the resistance genes were encoded on one large transferable plasmid. This report demonstrates the complexity of multiply resistant organisms.

PMID:
10511405
DOI:
10.1093/jac/44.3.377
[Indexed for MEDLINE]

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