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Appl Microbiol Biotechnol. 1999 Aug;52(2):170-3.

Acetate enhances solvent production and prevents degeneration in Clostridium beijerinckii BA101

Author information

1
Department of Food Science and Human Nutrition, University of Illinois, Urbana 61801, USA.

Abstract

Addition of sodium acetate to chemically defined MP2 medium was found to increase and stabilize solvent production by Clostridium beijerinckii BA101, a solvent-hyperproducing mutant derived from C. beijerinckii NCIMB 8052. C. beijerinckii BA101 demonstrated a greater increase in solvent production than C. beijerinckii NCIMB 8052 when sodium acetate was added to MP2 medium. In 1-l batch fermentations, C. beijerinckii BA101 produced 32.6 g/l total solvents, with butanol at 20.9 g/l, when grown in MP2 medium containing 60 mM sodium acetate and 8% glucose. To our knowledge, these values represent the highest solvent and butanol concentrations produced by a solventogenic Clostridium strain when grown in batch culture.

PMID:
10499256
DOI:
10.1007/s002530051504

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