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Biol Chem. 1999 Jul-Aug;380(7-8):953-9.

A role of poly (ADP-ribose) polymerase in NF-kappaB transcriptional activation.

Author information

1
Institute of Veterinary Biochemistry, University of Z├╝rich, Switzerland.

Abstract

The transcription factor NF-kappaB plays a critical role in immune and inflammatory responses. Here we show that poly (ADP ribose) polymerase (PARP) is required for specific NF-kappaB transcriptional activation in vivo. The activation of the HIV-LTR promoter and an NF-kappaB-dependent artificial promoter was drastically reduced in PARP (-/-) cells, independently of the signaling pathway through which NF-kappaB was induced. Furthermore NF-kappaB-dependent gene activation was restored in vivo by the expression of PARP in PARP (-/-) cells. Finally, we show that both NF-kappaB and PARP formed a stable immunoprecipitable nuclear complex. This interaction did not need DNA binding. Our results suggest that PARP is an important cofactor in the activation cascade of NF-kappaB-dependent target genes.

PMID:
10494847
DOI:
10.1515/BC.1999.118
[Indexed for MEDLINE]

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