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Trans R Soc Trop Med Hyg. 1999 May-Jun;93(3):335-6.

The effect of formalin fixation on the polymerase chain reaction characterization of Entamoeba histolytica.

Author information

1
Departamento de Medicina Experimental, Facultad de Medicina, UNAM, Mexico.

Abstract

Formalin fixation is the most common storage, transportation and preservation method for stool samples. However, fixation dramatically reduces our ability to extract from stool samples DNA that is a suitable template for polymerase chain reaction (PCR)-based diagnostic tests. In this study we evaluated the effects of formalin concentration and of the time stored in fixative on the success of PCR amplification. We found that the deleterious effects of formalin are both time and concentration dependent and may result from fragmentation of fixed DNA during its purification.

PMID:
10492774
DOI:
10.1016/s0035-9203(99)90045-7
[Indexed for MEDLINE]

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