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Free Radic Biol Med. 1999 Sep;27(5-6):554-9.

Superoxide release from interleukin-1B-stimulated human vascular cells: in situ electrochemical measurement.

Author information

1
Département de Pharmacologie, UMR 8604, Université René Descartes, Faculté de Médecine Necker, Paris, France. privat@ext.jussieu.fr

Abstract

Release of superoxide anion by cultured vascular cells was investigated with the use of selective microelectrodes. Local concentration of superoxide anion (O2*-) was followed by differential pulse amperometry on a carbon microfiber at 0.1 V/SCE. The oxidation current allows O2*- detection in the 10(-8) M concentration range without interference of the other major oxygen species. Interleukin-1beta-stimulated O2*- release that progressively increased to reach local concentrations at the cell membrane level of 76 +/- 11 nm 40-60 min after stimulation in human cord vein endothelial cells, and 131 +/- 18 nm 1-2 h after stimulation in internal mammary artery smooth muscle cells. In the two types of cells, the O2*- oxidation signal was suppressed in the presence of superoxide dismutase. Spontaneous O2*-release from unstimulated cells was undetectable. These results demonstrate that selective microelectrodes allow direct and real-time monitoring of local O2*- released from vascular endothelial as well as from smooth muscle cells submitted to an inflammatory stimulus.

PMID:
10490275
DOI:
10.1016/s0891-5849(99)00097-0
[Indexed for MEDLINE]

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