Format

Send to

Choose Destination
J Virol Methods. 1999 Aug;81(1-2):101-8.

Development of a monoclonal antibody blocking-ELISA for detection of antibodies against Maedi-Visna virus.

Author information

1
Laboratório Nacional de Investigação Veterinária, Dept. Virologia, Lisbon, Portugal. vir.Iniv@mail.telepac.pt

Abstract

A monoclonal antibody (MAb) blocking ELISA (Blck-ELISA) was developed to detect antibodies against Maedi-Visna virus (MVV) in sheep sera. The assay employs a MAb directed against the envelope protein p90 of the virus in a sandwich blocking procedure. To determine whether the MAb was a potential antibody for developing a Blck-ELISA, a collection of three hundred sera obtained from several sheep flocks known to be infected with MVV were used to examine the sensitivity of the Blck-ELISA. A total of 50 serum samples originating from a flock free of MVV were tested to assess the specificity of the assay. The results were compared with a commercial indirect ELISA (I-ELISA) and samples giving a conflicting or doubtful result were tested by immunoblot. The Blck-ELISA proved to be specific, sensitive and it showed high reproducibility and low variability.

PMID:
10488767
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center