Format

Send to

Choose Destination
Mol Cell. 1999 Aug;4(2):281-6.

The dinB gene encodes a novel E. coli DNA polymerase, DNA pol IV, involved in mutagenesis.

Author information

1
Division of Genetics and Mutagenesis, National Institute of Health Sciences, Tokyo, Japan.

Abstract

In Escherichia coli, the dinB gene is required for the SOS-induced lambda untargeted mutagenesis pathway and confers a mutator phenotype to the cell when the gene product is overexpressed. Here, we report that the purified DinB protein is a DNA polymerase. This novel E. coli DNA polymerase (pol IV) is shown to be strictly distributive, devoid of proofreading activity, and prone to elongate bulged (misaligned) primer/template structures. Site-directed mutagenesis experiments of dinB also demonstrate that the polymerase activity of DinB is required for its in vivo mutagenicity. Along with the sequence homologies previously found within the UmuC-like protein family, these results indicate that the uncovered DNA polymerase activity may be a common feature of all these homologous proteins.

PMID:
10488344
DOI:
10.1016/s1097-2765(00)80376-7
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center