Characterization of 3-methoxyflavones using fast-atom bombardment and collision-induced dissociation tandem mass spectrometry

Y L Ma; H V Heuvel; M Claeys

doi:10.1002/(SICI)1097-0231(19991015)13:19<1932::AID-RCM735>3.0.CO;2-W

Characterization of 3-methoxyflavones using fast-atom bombardment and collision-induced dissociation tandem mass spectrometry

Rapid Commun Mass Spectrom. 1999;13(19):1932-42. doi: 10.1002/(SICI)1097-0231(19991015)13:19<1932::AID-RCM735>3.0.CO;2-W.

Authors

Y L Ma¹, H V Heuvel, M Claeys

Affiliation

¹ University of Antwerp (UIA), Department of Pharmaceutical Sciences, Universiteitsplein 1, B-2610 Antwerp, Belgium.

Abstract

A mass spectrometric method based on the combined use of fast-atom bombardment (FAB), collision-induced dissociation (CID) and linked scanning at constant B/E has been used for the analysis of the fragmentation behavior of protonated 3-methoxyflavones (3-MFs). It is shown that several diagnostic ions such as (1,3)A (+) and (0,2)B (+) ions allow for an unambiguous localization of functions in the A- and B-rings and that the position of an additional methoxy group in the B-ring could be determined by a detailed analysis of the spectral patterns. Isomeric 3-MFs can be differentiated using this methodology. General fragmentation patterns of 3-MFs are discussed and plausible formation mechanisms of relatively abundant fragment ions are proposed.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Flavonoids / chemistry*
Isomerism
Molecular Structure
Spectrometry, Mass, Fast Atom Bombardment

Substances

Flavonoids