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J Allergy Clin Immunol. 1999 Sep;104(3 Pt 1):608-12.

Isolation and characterization of the mountain cedar (Juniperus ashei) pollen major allergen, Jun a 1.

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1
Department of Pediatrics, University of Texas Medical Branch at Galveston, 77555-0366, USA.

Abstract

BACKGROUND:

Cedar pollens are important causes of seasonal allergic disease in diverse geographic areas.

OBJECTIVE:

A major allergen from mountain cedar (Juniperus ashei) pollen, termed Jun a 1, was isolated and characterized.

METHODS:

Water-soluble pollen glycoproteins were extracted, salt precipitated, and purified with use of concanavalin A affinity chromatography or HPLC. The purified fractions were characterized by SDS-PAGE, immunoblotting, and N-terminal amino acid sequence analysis. Binding of allergen-specific IgE from the sera of cedar-hypersensitive patients was detected by ELISA and antigen-specific responses of peripheral blood T cells by tritiated thymidine incorporation.

RESULTS:

The major extractable cedar pollen glycoprotein had a molecular weight and N-terminal amino acid sequence that was similar to that of the major allergen Cha o 1, from Japanese cypress (Chamaecyparis obtusa), and Cry j 1, from Japanese cedar (Cryptomeria japonica). IgE from cedar-hypersensitive patients' sera bound to the isolated glycoprotein.

CONCLUSION:

The predominance of Jun a 1 in the soluble proteins of mountain cedar pollen and its high degree of homology with Cha o 1 and Cry j 1 make it likely to be the major allergen of this pollen. Amino acid sequence conservation also makes Jun a 1 a potential target for cross-reactivity between these pollen allergens. The observed reactivity of IgE from the sera of Japanese cedar-sensitive patients with Jun a 1 is consistent with this proposition.

PMID:
10482835
[Indexed for MEDLINE]
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