Format

Send to

Choose Destination
See comment in PubMed Commons below
Nucleic Acids Res. 1999 Oct 1;27(19):e27.

Gene targeting restricted to mouse striated muscle lineage.

Author information

1
Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), INSERM, CNRS, ULP, BP163, 67404 Illkirch cedex, C.U. de Strasbourg, France.

Abstract

Spatially and temporally regulated somatic mutations can be achieved by using the Cre/LoxP recombination system of bacteriophage P1. In order to develop gene knockouts restricted to striated muscle, we generated a transgenic mouse line expressing Cre recombinase under the control of the human alpha-skeletal actin promoter. Specific excision of a loxP-flanked gene was demonstrated in striated muscle, heart and skeletal muscle, in a pattern very similar to the expression of the endogenous alpha-skeletal actin gene. Therefore, the reported transgenic line can be used to target inactivation or activation of a given gene to the skeletal muscle lineage.

PMID:
10481039
PMCID:
PMC148637
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for PubMed Central
    Loading ...
    Support Center