Isolation and amino acid sequence of a peptide with vitellogenesis inhibiting activity from the terrestrial isopod Armadillidium vulgare (Crustacea)

P Grève; O Sorokine; T Berges; C Lacombe; A Van Dorsselaer; G Martin

doi:10.1006/gcen.1999.7330

Isolation and amino acid sequence of a peptide with vitellogenesis inhibiting activity from the terrestrial isopod Armadillidium vulgare (Crustacea)

Gen Comp Endocrinol. 1999 Sep;115(3):406-14. doi: 10.1006/gcen.1999.7330.

Authors

P Grève¹, O Sorokine, T Berges, C Lacombe, A Van Dorsselaer, G Martin

Affiliation

¹ Génétique et Biologie des Populations de Crustacés, UMR CNRS 6556.

PMID: 10480992
DOI: 10.1006/gcen.1999.7330

Abstract

The complete amino acid sequence of a neuropeptide was established using gas-phase microsequencing, mass spectrometry, and reverse transcription-polymerase chain reaction. This peptide, stored in the sinus gland of the terrestrial isopod Armadillidium vulgare, inhibited vitellogenin synthesis by the fat tissue and inhibited the onset of secondary vitellogenesis when tested in homologous bioassays. This peptide, named Arv-VIH, has 83 amino acid residues and a molecular mass of 9485 Da. Relationships with other related peptides are presented.

Publication types

Comparative Study

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Carrier Proteins / chemistry*
Carrier Proteins / genetics
Chromatography, High Pressure Liquid
Crustacea / metabolism*
Cyanogen Bromide
Female
Invertebrate Hormones / chemistry
Invertebrate Hormones / genetics
Male
Mass Spectrometry
Molecular Sequence Data
Neuropeptides / chemistry*
Neuropeptides / genetics
Peptide Fragments / chemistry
Sequence Homology
Trypsin
Vitellogenesis / drug effects*

Substances

Carrier Proteins
Invertebrate Hormones
Neuropeptides
Peptide Fragments
vitellogenesis inhibiting hormone
Trypsin
Cyanogen Bromide