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Nat Med. 1999 Sep;5(9):1052-6.

Genetic capsid modifications allow efficient re-targeting of adeno-associated virus type 2.

Author information

1
Laboratorium für Molekulare Biologie, Genzentrum Medizinische Klinik III, Klinikum Grobetahadern, Ludwig-Maximilians-Universität München, Feodor-Lynen-Strabetae 25, D-81377 München, Germany.

Erratum in

  • Nat Med 1999 Dec;5(12):1438.

Abstract

The human parvovirus adeno-associated virus type 2 (AAV2) has many features that make it attractive as a vector for gene therapy. However, the broad host range of AAV2 might represent a limitation for some applications in vivo, because recombinant AAV vector (rAAV)-mediated gene transfer would not be specific for the tissue of interest. This host range is determined by the binding of the AAV2 capsid to specific cellular receptors and/or co-receptors. The tropism of AAV2 might be changed by genetically introducing a ligand peptide into the viral capsid, thereby redirecting the binding of AAV2 to other cellular receptors. We generated six AAV2 capsid mutants by inserting a 14-amino-acid targeting peptide, L14, into six different putative loops of the AAV2 capsid protein identified by comparison with the known three-dimensional structure of canine parvovirus. All mutants were efficiently packaged. Three mutants expressed L14 on the capsid surface, and one efficiently infected wild-type AAV2-resistant cell lines that expressed the integrin receptor recognized by L14. The results demonstrate that the AAV2 capsid tolerates the insertion of a nonviral ligand sequence. This might open new perspectives for the design of targeted AAV2 vectors for human somatic gene therapy.

PMID:
10470084
DOI:
10.1038/12491
[Indexed for MEDLINE]

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