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Hum Gene Ther. 1999 Aug 10;10(12):2013-7.

A simple method for constructing E1- and E1/E4-deleted recombinant adenoviral vectors.

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1
Division of Biological Chemistry and Biologicals, National Institute of Health Sciences, Tokyo, Japan.

Abstract

We previously developed a two-plasmid in vitro ligation method that did not require a recombination step to produce new recombinant E1- or E1/E3-deleted adenoviral vectors. In this study, we have modified the system to improve the simplicity of vector construction and, in addition, to allow for production of an E1/E4-deleted vector.

PMID:
10466635
DOI:
10.1089/10430349950017374
[Indexed for MEDLINE]
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